Use of recombinant human parvovirus B19 antigens in serological assays.
نویسندگان
چکیده
منابع مشابه
Use of recombinant human parvovirus B19 antigens in serological assays.
AIMS--To compare the sensitivity, specificity, and practicality of recombinant proteins in serological tests for the detection of human parvovirus B19 IgG and IgM. METHODS--Indirect enzyme linked immunosorbent assays using B19 structural proteins expressed in Escherichia coli were developed for the detection of B19 specific IgG and IgM (rELISA-G and rELISA-M). Cells infected with baculovirus ex...
متن کاملUndenatured parvovirus B19 antigens are essential for the accurate detection of parvovirus B19 IgG.
Recombinant versions of parvovirus B19 capsid proteins VP1 and VP2 are used for immunodiagnostic assays for detection of antiviral antibodies. The immune response to B19 is characterized by a gradual loss of antibodies directed against linear epitopes of VP2. A similar occurrence for antibodies raised against VP1 protein would represent a limitation to serological assays incorporating denatured...
متن کاملSerological prevalence of human parvovirus B19 in diseases or disordersrelated to different human body systems.
BACKGROUND/AIM Human parvovirus B19 is a pathogen that affects different parts of the body. We planned this study because of the lack of data on B19 seroprevalence based on different body-system diseases. MATERIALS AND METHODS The prevalence of parvovirus B19 antibodies was investigated retrospectively in 1239 patients by review of medical records from 2009-2012, according to their diseases c...
متن کاملDiagnostic and prognostic value of molecular and serological investigation of human parvovirus B19 infection during pregnancy.
To define diagnostic and prognostic markers of parvovirus B19 (B19V) fetal infection, two groups were investigated: 1) pregnant women with specific symptoms or contacts with symptomatic households (n=37); 2) mothers with pathological ultrasound findings and the relevant fetus at the time of prenatal diagnosis (n=16). In the first group, diagnosis of B19V infection was achieved using IgM detecti...
متن کاملmiRNA and tropism of human parvovirus B19
Parvovirus B19 has an extreme tropism for human erythroid progenitors. Here we propose the hypothesis explaining the tropism of human parvovirus B19. Our speculations are based on experimental results related to the capsid proteins VP1 and VP2. These proteins were not detectable in nonpermissive cells in course of these experiments, although the corresponding mRNAs were synthesized. Our interpr...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1993
ISSN: 0021-9746
DOI: 10.1136/jcp.46.9.840